Journal: JCI Insight
Article Title: Characterization of the clonal hierarchy and immunophenotype of PTPN11 mutations in acute myeloid leukemia
doi: 10.1172/jci.insight.193779
Figure Lengend Snippet: ( A ) Overall survival of Npm1 cA , Ptpn11 E76K , and Npm1 cA / Ptpn11 E76K mice. ( B ) Representative picture of a spleen collected from a Npm1 cA / Ptpn11 E76K mouse at survival endpoint compared with age-matched Mx-Cre mouse spleen. Summary of spleen weights (in grams) of Npm1 cA / Ptpn11 E76K mice at time of death compared with aged-matched controls. ( C ) White blood cell (WBC), red blood cell (RBC), and platelet (PLT) counts of Npm1 cA / Ptpn11 E76K mice at time of death compared with aged-matched controls. ( D ) Representative peripheral blood flow cytometry plot (gated on CD45 + CD3 – CD19 – NK1.1 – cells; percentage based on total CD45 + cells) of Mx-Cre and Npm1 cA / Ptpn11 E76K mice 4 weeks after poly(I:C) induction (total n of 11 Mx-Cre mice and 14 Ptpn11 E76K / Npm1 cA mice). Data are presented as mean ± SD. Statistical analysis by 1-way ANOVA with Benjamini-Hochberg FDR correction applied. *FDR-adjusted P ≤ 0.05, ***FDR-adjusted P ≤ 0.001, ****FDR-adjusted P ≤ 0.0001.
Article Snippet: Cryopreserved samples were thawed and counted using the Cellaca PLX Image Cytometry System (Nexcelom).
Techniques: Flow Cytometry